Journal: Diagnostic Pathology
Article Title: A comparative proteomic study identified calreticulin and prohibitin up-regulated in adrenocortical carcinomas
doi: 10.1186/1746-1596-8-58
Figure Lengend Snippet: Representative images of immunohistochemical results. Expression of calreticulin, prohibitin and HSP60 in adrenocortical carcinomas (ACC), adrenocortical adenomas (ACA) and normal tissues (N) ( A ). Calreticulin and prohibitin but not HSP60 were overexpressed in ACC compared with adrenocortical adenomas (ACA) and normal tissues ( B ).
Article Snippet: Frozen ACC and normal adjacent adrenocortical tissues were first homogenized using a sample grinding kit (GE Healthcare) with a lysis buffer (7 M urea, thiourea 2 M, 2% CHAPS, 18 mM DTT, 1 mM PMSF, 0.5% IPG buffer pH3~10NL), and then the extracts were centrifuged at 12,000 g, 4°C, for 1 hr.
Techniques: Immunohistochemical staining, Expressing